VersaFlour 荧光分光光度计是一款灵活而且实用的台式荧光分光光度计，用户能自选滤光片来精确控制激发光和发射光波长。VersaFlour 荧光分光光度计可激发和检测350–900nm 范围内的任何荧光染料。使用简便的菜单式软件使你既可以人工测试每个样品，也可自动记录动力学分析。量程和增益可调，以获得最大的灵敏度。VersaFlour 荧光分光光度计与一次性比色杯、标准石英比色杯及微型比色杯匹配。通过RS-232串口将数据输送至相应的热敏打印机。
使用Hoechst 染料的标准2ml 检测，VersaFlour 荧光分光光度计可对浓度低至10 ng/ml 的DNA 样品精确定量。 当RNA 蛋白质和核苷酸存在时，Hoechst 染料可选择性地与dsDNA 结合，从而获得高灵敏度和高选择性的结果， 足以满足实时定量PCR 和确定拷贝数的要求。VersaFlour 荧光分光光度计：
- 快速、精确地定量DNA、RNA 寡核苷酸和蛋白质
- 用于实时定量PCR 分析中的拷贝数确定
- 报告基因分析（通过测定beta半乳糖苷酶和beta葡糖苷酸酶活性，绿色荧光蛋白）
- 细胞（死亡）凋亡研究
使用Bio-Rad 标准比色杯（1.0–3.5 ml）或独特的小容量微型比色杯（150–300µl）可避免样品与样品之间的交叉污染。
用户可选择的滤光片具有大的激发光和发射光波长范围，适合多数应用领域。一系列优化的滤光片产品可用于蛋白和核酸定量、beta- 半乳糖苷酶和beta- 葡糖苷酸酶的各种测定中。您也可与当地的Bio-Rad 销售代表联系来定制滤光片。下表列有常用的荧光检测方法和所需的滤光片。只需根据你的测试要求选择合适的激发光和发射光滤光片即可。
DNA 定量试剂盒
Bio-Rad DNA 荧光定量试剂盒可快速而精确地定量溶液中的双链DNA——即使有RNA 和蛋白存在。DNA 被二苯甲亚胺染料（Hoechst 33258）定量染色，这种染料的激发波长为346 nm，发射波长为460 nm。这种蓝光能很容易地被VersaFluor 或其它荧光分光光度计检测到。试剂盒可定量10 ng/ml -5 µg/ml 的dsDNA，每个包装可进行 750 个2 ml 标准反应。
FluorAce beta-半乳糖苷酶报告基因检测试剂盒
FluorAce beta- 半乳糖苷酶报告基因检测试剂盒可简单、精确地检测beta- 半乳糖苷酶活性。只需将荧光底物4- methylumbelliferyl beta-D- 吡喃型半乳糖酶（MUG）加入到细胞培养液中即可。底物进入细胞后被beta- 半乳糖苷酶剪切，产生的荧光化合物4-methylumbelliferone (4 MU) 能容易地被VersaFlour 或其它荧光分光光度计检测。每个包装可进行200 个1.5ml 标准反应。
FluorAce beta-葡糖苷酸酶报告基因检测试剂盒
FluorAce beta- 葡糖苷酸酶报告基因检测盒可快速精确地检测β-葡糖苷酸酶活性。底物4-methylumbelliferyl beta-D- 葡糖苷酸（MUGluc）被beta-葡糖苷酸酶剪切，产生的荧光化合物能容易地被VersaFlour 或其它荧光分光光度计检测。每个包装可进行200 个1.5ml 标准反应。
| 应用 | 激发光滤光片 | 货号＃ | 发射光滤光片 | 货号＃ |
| DNA 定量(Hoechst 33258) | EX 360/40 (340–380 nm) |
170-2420 | EM 460/10 (455–465 nm) |
170-2421 |
| beta-半乳糖苷酶(MUG) | EX 360/40 (340–380 nm) |
170-2420 | EM 460/10 (455–465 nm) |
170-2421 |
| beta-半乳糖苷酶(MUGluc) | EX 360/40 (340–380 nm) |
170-2420 | EM 460/10 (455–465 nm) |
170-2421 |
| DNA 定量(PicoGreen) | EX 480/20 (470–490 nm) |
170-2427 | EM 520/10 (515–525 nm) |
170-2424 |
| RNA 定量(RiboGreen) | EX 490/10 (485–495 nm) |
170-2422 | EM 520/10 (515–525 nm) |
170-2424 |
| 寡核苷酸/蛋白定量 (OliGreen) |
EX 480/20 (470–490 nm) |
170-2427 | EM 520/10 (515–525 nm) |
170-2424 |
| 蛋白定量(NanoOrange) | EX 480/20 (470–490 nm) |
170-2427 | EM 590/10 (585–595 nm) |
170-2425 |
| 荧光素 | EX 490/10 (485–495 nm) |
170-2422 | EM 520/10 (515–525 nm) |
170-2424 |
| 溴化乙锭 | EX 510/10 (505–515 nm) |
170-2423 | EM 620/10 (615–625 nm) |
170-2426 |
| wtGFP (野生型Green Fluorescent Protein) |
EX 390/22 (379–401 nm) |
170-2443 | EM 510/10 (505–515 nm) |
170-2445 |
| EGFP (红移Green Fluorescent Protein) |
EX 490/10 (485–495 nm) |
170-2422 | EM 510/10 (505–515 nm) |
170-2445 |
| EBFP (发射蓝光Green Fluorescent Protein) |
EX 380/30 (365–395 nm) |
170-2444 | EM 440/10 (435–445 nm) |
170-2446 |
| GFPuv GFPuv (紫外激发Green Fluorescent Protein) |
EX 390/22 (379–401 nm) |
170-2443 | EM 510/10 (505–515 nm) |
170-2445 |
| 目录 # | 描述 |
| VersaFluor Fluorometer | |
| 170-2402 | VersaFluor Fluorometer, 100/120/220 V, includes one standard cuvette holder, 100 standard cuvettes, choice of 1 excitation and 1 emission filter |
| 170-2403 | VersaFluor Fluorometer System, 120 V, includes one standard cuvette holder, 100 standard cuvettes, and choice of one excitation and one emission filter, plus a 120 V thermal printer and cable |
| 170-2404 | VersaFluor Fluorometer System, 100 V, includes everything listed in 170-2402, plus a 100 V thermal printer and cable |
| VersaFluor Disposable Cuvettes | |
| 170-2415 | Standard Cuvettes, 4-sided, optically clear polystyrene, 3.5 ml, 100 |
| 170-2416 | Microcuvette, 3.0 x 3.0 mm (outside dimensions), 4-sided optically clear polystyrene, 150–350 µl, 100 |
| Filter Sets (Excitation and Emission) | |
| 170-2435 | Filter Set (EX 360/40, EM 460/10), for DNA quantitation (Hoechst 33258) and beta-galactosidase (MUG) and beta-glucuronidase (MUGluc) assays |
| 170-2436 | Filter Set (EX 490/10, EM 520/10), for fluorescein-based assays |
| 170-2437 | Filter Set (EX 360/40, EM 520/10) |
| 170-2447 | Filter Set (EX 390/2, EM 510/10), for wild-type GFP and UV-excited GFP |
| Individual Emission Filters | |
| 170-2421 | EM 460/10 (455–465 nm) |
| 170-2424 | EM 520/10 (515–525 nm) |
| 170-2425 | EM 590/10 (585–595 nm) |
| 170-2426 | EM 620/10 (615–625 nm) |
| 170-2445 | EM 510/10 (505–515 nm) |
| 170-2446 | EM 440/10 (435–445 nm) |
| Individual Excitation Filters | |
| 170-2420 | EX 360/40 (340–380 nm) |
| 170-2422 | EX 490/10 (485–495 nm) |
| 170-2423 | EX 510/10 (505–515 nm) |
| 170-2427 | EX 480/20 (470–490 nm) |
| 170-2443 | EX 390/22 (379–401 nm) |
| 170-2444 | EX 380/30 (365–395 nm) |
| Accessories | |
| 170-2406 | Standard Cuvette Holder |
| 170-2407 | Microcuvette Holder |
| 170-2430 | Quartz Halogen Replacement Lamp |
| 170-2450 | Optical Filter Storage Case |
| Printers | |
| 170-2409 | VersaFluor Thermal Printer, 100 V, with cable and power adaptor |
| 170-2410 | VersaFluor Thermal Printer, 120 V, with cable and power adaptor |
| 170-2411 | VersaFluor Thermal Printer, 220 V, with cable and power adaptor |
| 170-2412 | Thermal Printer Paper, 10 rolls |
| 170-2413 | Thermal Printer Universal Battery Pack |
